There are some days when things just don’t seem to go as planned, and then there are other days when the lab reagents are just out to get you. Yesterday was one of the latter days! Yesterday I was making (trying to make) an SDS gel.
This isn’t the first time I’ve done SDS-PAGE. In the past I’ve had some lovely gels. In the past I’ve also had some gels that were truly disastrous! I have no idea what it was I did differently.
My gel yesterday: the resolving gel set in the tube I made it in really well – just not very well between the glass plates (and mystery air bubbles appeared when I know they weren’t there when I poured it); the stacking gel set between the plates but not in the tube.
It has reached the point where I now walk away from the gel leaving it to set as one has never set whilst I’ve been in the same room as it (I’m going with the theory of the watched pot etc.)
Has anyone out there got a foolproof technique for pouring these things? Otherwise I’m going to have to carry on crossing my fingers, leaving the room, and trusting to luck…